Journal: Movement Disorders

Article Title: An X‐Linked Ataxia Syndrome in a Family with Hearing Loss Associated with a Novel Variant in the BCAP31 Gene

doi: 10.1002/mds.30116

Figure Lengend Snippet: Subcellular localization of mutant (mut) BCAP31. ( A ) Subcellular localization of wild‐type (WT) and V8I BCAP31 was investigated using confocal laser scanning microscopy (CLSM) imaging. Results shows BCAP31‐tGFP and ER‐mRFP (top); BCAP31 mut‐tGFP and endoplasmic reticulum (ER)‐mRFP (bottom) co‐localization in the live SH‐SY5Y cells (scale bar: 10 μm). ( B ) Corresponding quantification of colocalization by the Mander's overlap coefficient (MOC) and the Pearson correlation coefficient (PCC). n = 34 cells. Unpaired t test (MOC) and Kolmogorov–Smirnov test (PCC). [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Confocal laser scanning microscopy (CLSM) imaging of live cells expressing BCAP31 genetically fused with tGFP, was performed using the Zeiss Airyscan 880 inverted microscope system equipped with an Ar‐ion laser (with three lines: 458 nm, 488 nm, and 514 nm) and a 633 nm He‐Ne laser, a Plan Apochromat 40/1.4 oil immersion objective, main dichroic beam splitter 488/561/633, emission filters BP 493 to 598 nm (turbo green fluorescent protein (tGFP)), and BP 580‐620 nm (monomeric red fluorescent protein (mRFP)), and GaAsP PMT detector.

Techniques: Mutagenesis, Confocal Laser Scanning Microscopy, Imaging